Fluorescence and transmission microscopy are noninvasive methods ideally suited to resolve the dynamical processes in microbial communities that will allows us to observe spatially structured communities using rRNA fluorescent in situ hybridization (FISH) as well as fluorescent or fluorogenic probes for visualizing small molecules.
Using our Leica Laser Microdissection Microscope (LMD7), you can isolate specific cells, mucus layers, inflammatory foci or cancers from histology slides. You can use your already established tissue banks to generate new data, or through our services you can access to UW Medicine biorepositories.
Leica Laser Microdissection Microscope (LMD7)
The Leica Laser Microdissection Microscope is a contact- and contamination-free method for isolating specific single cells or entire areas of tissue from a wide variety of tissue samples. The samples can then be available for further molecular biological methods such as PCR, real-time PCR, proteomics and genomics.
Leica Fluorescence Microscope (DM4)
Generate or acquire fluorescent probes against specific bacterial members of the microbiota in order to visualize their relationship with each other as well as with the intestinal tissue using fluorescent microscopy.
Leica Laser Microdissection Microscope
Ecology and community are essential and important aspects to the microbiome and cannot be truly understood using computational models and synthetic biology. Therefore, imaging the microbiome will be a critical tool to get us past the descriptive and theoretical science that is so common today.